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Promag Progressers Facility Services Pvt Ltd diameter promag beads
Diameter Promag Beads, supplied by Promag Progressers Facility Services Pvt Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diameter+promag+beads/streptavidin+coated+superparamagnetic+bead+promag+beads/us10870111-242-20-21
Average 90 stars, based on 1 article reviews
diameter promag beads - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Fluidic devices with bead well geometries with spatially separated bead retention and signal detection segments and related methods
Article Snippet: .. FIG. 8C is an image illustrating a high background fluorescence of the beads in an array loaded with 3.3 μm diameter Promag beads. ..



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90
Promag Progressers Facility Services Pvt Ltd diameter promag beads
Diameter Promag Beads, supplied by Promag Progressers Facility Services Pvt Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diameter+promag+beads/streptavidin+coated+superparamagnetic+bead+promag+beads/us10870111-242-20-21
Average 90 stars, based on 1 article reviews
diameter promag beads - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Bangs Laboratories promag™ carboxylic mpio (0.76 μm diameter, 2.114 x 10 9 particles/mg bead, iron content: 26%; cat. no. pmc1n, bangs laboratory inc., usa)
<t>ALCAM-MPIO</t> construct and its binding ability in vitro. (a–c) Immunofluorescent images showing binding of R&D ALCAM-MPIO (red box) (a) or IgG-MPIO (green box) (b) on TNF stimulated mouse endothelial cells; red = MPIO, green = ALCAM and blue = DAPI. Scale bars = 10 µm. (c) Graph showing percentage of co-localized red (MPIO) and green (ALCAM) pixels for each FoV; R&D ALCAM-MPIO showed significantly higher binding to endothelial cell ALCAM than IgG-MPIO; *** p <0.001. (d–g) Fluorescently labelled ALCAM-MPIO (d) or IgG-MPIO (e) were pumped through glass capillaries coated with ALCAM protein, or ALCAM-MPIO were pumped through glass capillaries coated with control BSA protein (f). Only binding of ALCAM-MPIO to ALCAM coated capillaries was evident (d). (g) Graph showing number of ALCAM- or IgG-MPIO bound to the capillary wall; ALCAM-MPIO binding to ALCAM coated capillaries was significantly higher than either IgG-MPIO in ALCAM coated capillaries or ALCAM-MPIO in BSA coated capillaries. **** p <0.0001.
Promag™ Carboxylic Mpio (0.76 μm Diameter, 2.114 X 10 9 Particles/Mg Bead, Iron Content: 26%; Cat. No. Pmc1n, Bangs Laboratory Inc., Usa), supplied by Bangs Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diameter+promag+beads/magnetic+beads/pmc08217895-54-8-25
Average 90 stars, based on 1 article reviews
promag™ carboxylic mpio (0.76 μm diameter, 2.114 x 10 9 particles/mg bead, iron content: 26%; cat. no. pmc1n, bangs laboratory inc., usa) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


ALCAM-MPIO construct and its binding ability in vitro. (a–c) Immunofluorescent images showing binding of R&D ALCAM-MPIO (red box) (a) or IgG-MPIO (green box) (b) on TNF stimulated mouse endothelial cells; red = MPIO, green = ALCAM and blue = DAPI. Scale bars = 10 µm. (c) Graph showing percentage of co-localized red (MPIO) and green (ALCAM) pixels for each FoV; R&D ALCAM-MPIO showed significantly higher binding to endothelial cell ALCAM than IgG-MPIO; *** p <0.001. (d–g) Fluorescently labelled ALCAM-MPIO (d) or IgG-MPIO (e) were pumped through glass capillaries coated with ALCAM protein, or ALCAM-MPIO were pumped through glass capillaries coated with control BSA protein (f). Only binding of ALCAM-MPIO to ALCAM coated capillaries was evident (d). (g) Graph showing number of ALCAM- or IgG-MPIO bound to the capillary wall; ALCAM-MPIO binding to ALCAM coated capillaries was significantly higher than either IgG-MPIO in ALCAM coated capillaries or ALCAM-MPIO in BSA coated capillaries. **** p <0.0001.

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models

doi: 10.1177/0271678X20968943

Figure Lengend Snippet: ALCAM-MPIO construct and its binding ability in vitro. (a–c) Immunofluorescent images showing binding of R&D ALCAM-MPIO (red box) (a) or IgG-MPIO (green box) (b) on TNF stimulated mouse endothelial cells; red = MPIO, green = ALCAM and blue = DAPI. Scale bars = 10 µm. (c) Graph showing percentage of co-localized red (MPIO) and green (ALCAM) pixels for each FoV; R&D ALCAM-MPIO showed significantly higher binding to endothelial cell ALCAM than IgG-MPIO; *** p <0.001. (d–g) Fluorescently labelled ALCAM-MPIO (d) or IgG-MPIO (e) were pumped through glass capillaries coated with ALCAM protein, or ALCAM-MPIO were pumped through glass capillaries coated with control BSA protein (f). Only binding of ALCAM-MPIO to ALCAM coated capillaries was evident (d). (g) Graph showing number of ALCAM- or IgG-MPIO bound to the capillary wall; ALCAM-MPIO binding to ALCAM coated capillaries was significantly higher than either IgG-MPIO in ALCAM coated capillaries or ALCAM-MPIO in BSA coated capillaries. **** p <0.0001.

Article Snippet: To construct ALCAM-MPIO, antibodies were conjugated to ProMag™ carboxylic MPIO (0.76 μm diameter, 2.114 x 10 9 particles/mg bead, iron content: 26%; cat. no. PMC1N, Bangs Laboratory Inc., USA), as described previously.

Techniques: Construct, Binding Assay, In Vitro

ALCAM-MPIO specificity assessment in naïve mice. (a) Representative T 2 *-weighted MGE3D coronal images from naïve mice injected with either (i) untagged ProMag™ carboxylic MPIO, (ii) IgG-MPIO, or (iii) ALCAM-MPIO. Negligible hypointense voxels arising from MPIO were detected in these control group. (b) Representative T 2 *-weighted MGE3D coronal images from tumour bearing mice injected with non-specific IgG-MPIO on day 21 (MDA231Br-GFP, H1_DL2) and day 42 (SEBTA-001). Again, minimal hypointense voxels were detected in these mice.

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models

doi: 10.1177/0271678X20968943

Figure Lengend Snippet: ALCAM-MPIO specificity assessment in naïve mice. (a) Representative T 2 *-weighted MGE3D coronal images from naïve mice injected with either (i) untagged ProMag™ carboxylic MPIO, (ii) IgG-MPIO, or (iii) ALCAM-MPIO. Negligible hypointense voxels arising from MPIO were detected in these control group. (b) Representative T 2 *-weighted MGE3D coronal images from tumour bearing mice injected with non-specific IgG-MPIO on day 21 (MDA231Br-GFP, H1_DL2) and day 42 (SEBTA-001). Again, minimal hypointense voxels were detected in these mice.

Article Snippet: To construct ALCAM-MPIO, antibodies were conjugated to ProMag™ carboxylic MPIO (0.76 μm diameter, 2.114 x 10 9 particles/mg bead, iron content: 26%; cat. no. PMC1N, Bangs Laboratory Inc., USA), as described previously.

Techniques: Injection

Detection of brain micrometastases using ALCAM-MPIO. (a) Representative T 2 *-weighted coronal images from MGE3D datasets. Prominent focal hypointense (black) foci corresponding to retention of ALCAM-MPIO were evident in mice injected with MDA231Br-GFP or H1_DL2 cells at both days 21 and 28, and SEBTA-001 cells at day 42. Examples of hypointense foci are indicated with white arrows in the first column. (b) Graph showing quantitation of the number of hypointense voxels for each tumor model (n = 4–8 per group). A significant difference was evident between tumor bearing mice injected with ALCAM-MPIO (black circles) and IgG-MPIO (grey squares) for all time-points in all three models; * p <0.05, ** p <0.01, *** p <0.001. Dashed line denotes background hypointense voxels detected in naive mice injected with ALCAM-MPIO (n = 2).

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models

doi: 10.1177/0271678X20968943

Figure Lengend Snippet: Detection of brain micrometastases using ALCAM-MPIO. (a) Representative T 2 *-weighted coronal images from MGE3D datasets. Prominent focal hypointense (black) foci corresponding to retention of ALCAM-MPIO were evident in mice injected with MDA231Br-GFP or H1_DL2 cells at both days 21 and 28, and SEBTA-001 cells at day 42. Examples of hypointense foci are indicated with white arrows in the first column. (b) Graph showing quantitation of the number of hypointense voxels for each tumor model (n = 4–8 per group). A significant difference was evident between tumor bearing mice injected with ALCAM-MPIO (black circles) and IgG-MPIO (grey squares) for all time-points in all three models; * p <0.05, ** p <0.01, *** p <0.001. Dashed line denotes background hypointense voxels detected in naive mice injected with ALCAM-MPIO (n = 2).

Article Snippet: To construct ALCAM-MPIO, antibodies were conjugated to ProMag™ carboxylic MPIO (0.76 μm diameter, 2.114 x 10 9 particles/mg bead, iron content: 26%; cat. no. PMC1N, Bangs Laboratory Inc., USA), as described previously.

Techniques: Injection, Quantitation Assay

MRI BBB permeability assessment in the brain metastasis models. (Top three rows) Representative T 2 *-weighted MGE3D images and corresponding gold standard T 1 -weighted pre- and post-gadolinium images for mice injected intracardially with MDA-MB-231-Br, H1_DL2 or SEBTA-001 cells. No gadolinium enhancement (bright regions) was detected in T 1 -weighted post-Gd images, indicating an intact BBB. The presence of hypointense foci on the T 2 *-weighted MGE3D correspond to the bound ALCAM-MPIO and micrometastases, which are not detected on the post-contrast T 1 -weighted image. Images are from the latest time-point of the study (day 28 post-injection for MDA-MB-231-Br, H1_DL2 and day 42 for SEBTA-001).

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models

doi: 10.1177/0271678X20968943

Figure Lengend Snippet: MRI BBB permeability assessment in the brain metastasis models. (Top three rows) Representative T 2 *-weighted MGE3D images and corresponding gold standard T 1 -weighted pre- and post-gadolinium images for mice injected intracardially with MDA-MB-231-Br, H1_DL2 or SEBTA-001 cells. No gadolinium enhancement (bright regions) was detected in T 1 -weighted post-Gd images, indicating an intact BBB. The presence of hypointense foci on the T 2 *-weighted MGE3D correspond to the bound ALCAM-MPIO and micrometastases, which are not detected on the post-contrast T 1 -weighted image. Images are from the latest time-point of the study (day 28 post-injection for MDA-MB-231-Br, H1_DL2 and day 42 for SEBTA-001).

Article Snippet: To construct ALCAM-MPIO, antibodies were conjugated to ProMag™ carboxylic MPIO (0.76 μm diameter, 2.114 x 10 9 particles/mg bead, iron content: 26%; cat. no. PMC1N, Bangs Laboratory Inc., USA), as described previously.

Techniques: Permeability, Injection

ALCAM-MPIO binding and detection of brain metastases. (a–c) Representative T 2 *-weighted MGE3D image from MDA-MB-231-Br brain metastasis model and the corresponding histological section (b) showing an example of co-localisation of hypointensities on the T 2 *-weighted image with brain metastases (boxes) and (c) the higher magnification photomicrograph from ALCAM (brown) stained tumour. Counterstain = cresyl violet; blue. (d–f) Representative T 2 *-weighted MGE3D image from the H1_DL2 brain metastasis model and the corresponding histological sections. (g–i) Representative T 2 *-weighted MGE3D image from SEBTA-001 brain metastasis model and the corresponding histological sections. (j) Perls’ Prussian blue staining of tumour showing presence of MPIO (blue; arrows), counterstain = Nuclear Fast Red. (k and l) MPIO (black arrows) were found in ALCAM activated vessels (brown); nuclei of tumour cells are evident in close association with ALCAM-positive vessels. Scale bars: (b, e, h) = 1 mm; (c, f, I, j) = 30 µm; (e, f) = 5 µm.

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models

doi: 10.1177/0271678X20968943

Figure Lengend Snippet: ALCAM-MPIO binding and detection of brain metastases. (a–c) Representative T 2 *-weighted MGE3D image from MDA-MB-231-Br brain metastasis model and the corresponding histological section (b) showing an example of co-localisation of hypointensities on the T 2 *-weighted image with brain metastases (boxes) and (c) the higher magnification photomicrograph from ALCAM (brown) stained tumour. Counterstain = cresyl violet; blue. (d–f) Representative T 2 *-weighted MGE3D image from the H1_DL2 brain metastasis model and the corresponding histological sections. (g–i) Representative T 2 *-weighted MGE3D image from SEBTA-001 brain metastasis model and the corresponding histological sections. (j) Perls’ Prussian blue staining of tumour showing presence of MPIO (blue; arrows), counterstain = Nuclear Fast Red. (k and l) MPIO (black arrows) were found in ALCAM activated vessels (brown); nuclei of tumour cells are evident in close association with ALCAM-positive vessels. Scale bars: (b, e, h) = 1 mm; (c, f, I, j) = 30 µm; (e, f) = 5 µm.

Article Snippet: To construct ALCAM-MPIO, antibodies were conjugated to ProMag™ carboxylic MPIO (0.76 μm diameter, 2.114 x 10 9 particles/mg bead, iron content: 26%; cat. no. PMC1N, Bangs Laboratory Inc., USA), as described previously.

Techniques: Binding Assay, Staining

Classification accuracy of  ALCAM-MPIO  imaging in metastasis models.

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: A novel molecular magnetic resonance imaging agent targeting activated leukocyte cell adhesion molecule as demonstrated in mouse brain metastasis models

doi: 10.1177/0271678X20968943

Figure Lengend Snippet: Classification accuracy of ALCAM-MPIO imaging in metastasis models.

Article Snippet: To construct ALCAM-MPIO, antibodies were conjugated to ProMag™ carboxylic MPIO (0.76 μm diameter, 2.114 x 10 9 particles/mg bead, iron content: 26%; cat. no. PMC1N, Bangs Laboratory Inc., USA), as described previously.

Techniques: Imaging